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🌾🌾Dr.Agrarian🌾🌾 on Instagram: "Ethidium bromide (EtBr) is an intercalating fluorescent dye used in DNA analysis, especially in agarose gel electrophoresis. Main function EtBr inserts between base pairs of double-stranded DNA. When exposed to UV light, the EtBr–DNA complex fluoresces orange, allowing visualization of DNA bands in the gel. Purpose in DNA analysis Detect and locate DNA fragments after electrophoresis. Estimate DNA size and quantity by comparing band intensity with a DNA ladder.
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🌾🌾Dr.Agrarian🌾🌾 on Instagram: "Ethidium bromide (EtBr) is an intercalating fluorescent dye used in DNA analysis, especially in agarose gel electrophoresis. Main function EtBr inserts between base pairs of double-stranded DNA. When exposed to UV light, the EtBr–DNA complex fluoresces orange, allowing visualization of DNA bands in the gel. Purpose in DNA analysis Detect and locate DNA fragments after electrophoresis. Estimate DNA size and quantity by comparing band intensity with a DNA ladder.
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Future Biotechnology on Instagram: "Gel electrophoresis is a molecular biology technique used to separate nucleic acids (DNA or RNA) or proteins according to their molecular size and charge. In this process, a semi-solid agarose gel matrix acts as a molecular sieve. DNA samples, which are negatively charged due to their phosphate backbone, are loaded into wells and subjected to an electric field. The fragments migrate toward the positive (anode) end, with smaller DNA molecules moving faster thro
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